实例探究 > Scaling Up qPCR Assays with a Flexible and User-Friendly Automation Software Platform

Scaling Up qPCR Assays with a Flexible and User-Friendly Automation Software Platform

公司规模
Large Corporate
地区
  • Europe
国家
  • United Kingdom
产品
  • Antha
  • Gilson PIPETMAX
技术栈
  • Automation Software
  • Liquid Handling Robots
  • In Silico Simulation
实施规模
  • Enterprise-wide Deployment
影响指标
  • Productivity Improvements
  • Customer Satisfaction
  • Digital Expertise
技术
  • 分析与建模 - 预测分析
  • 功能应用 - 远程监控系统
适用行业
  • 生命科学
  • 医疗保健和医院
适用功能
  • 质量保证
  • 产品研发
用例
  • 预测性维护
  • 机器状态监测
  • 过程控制与优化
服务
  • 系统集成
  • 软件设计与工程服务
关于客户
The Cell and Gene Therapy Catapult, established in 2012, aims to build a world-leading cell and gene therapy sector in the UK. The organization supports companies of all sizes in developing and delivering cell and gene therapies (CGTs) to patients. With over 330 experts, the Catapult operates from state-of-the-art laboratories at Guy’s Hospital in London, a £70-million GMP-compliant manufacturing center in Stevenage, and a newly announced facility in Braintree. The Industrialization team focuses on improving CGT production efficiency in a cost-effective and scalable manner, conducting in-house research, and developing custom, integrated solutions for clients.
挑战
Routine analytical assays in the cell and gene therapy sector often require repetitive and complex manual actions, which can be time-consuming and prone to human error. The Cell and Gene Therapy Catapult needed a solution to automate these processes, particularly for qPCR assays, to increase efficiency, data reproducibility, and walkaway time for scientists. Traditional automation solutions lacked flexibility and required advanced programming skills, creating a high barrier to entry for many biologists.
解决方案
The Cell and Gene Therapy Catapult collaborated with Synthace in 2018 to automate qPCR assays using Synthace’s software platform Antha. Antha allows scientists to perform scalable and flexible qPCR assays on automated liquid handlers through a codeless, user-friendly interface. This platform increases experimental throughput, data reproducibility, and walkaway time for scientists. Antha standardizes protocols, removes variability between runs and operators, and minimizes human error, preserving data integrity. The platform also enables in silico simulation and optimization of assays before execution, ensuring efficient and error-free workflows.
运营影响
  • Antha enabled scientists to execute flexible and robust qPCR workflows on automated liquid handlers without programming knowledge.
  • The platform retained data integrity and high data quality, standardizing protocols and ensuring data reproducibility.
  • Scientists could simulate and optimize their assays in silico before execution on hardware, reducing hands-on time by up to 5 hours.
  • Antha minimized manual intervention, preventing human error and preserving data integrity.
  • The platform allowed for easy scalability of qPCR assays, providing consistent performance comparable to manual execution.
数量效益
  • Reduced hands-on time by up to 5 hours per week.
  • qPCR efficiency between 90-110% and less than 0.5Ct range between technical replicates.
  • Antha set up a 384-well plate in 1 hour 45 minutes compared to 5 hours manually.

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