Oxford Biomedica and Antha: Multifactorial Optimisation of a Lentiviral Vector Transfection/Transduction System
Company Size
1,000+
Region
- Europe
Country
- United Kingdom
Product
- LentiVector® platform
- Antha
- Synthace
- Gilson PIPETMAX®
Tech Stack
- Design of Experiments (DoE)
- Liquid Handling Automation
- In Silico Simulation
Implementation Scale
- Departmental Deployment
Impact Metrics
- Cost Savings
- Innovation Output
- Productivity Improvements
Technology Category
- Analytics & Modeling - Predictive Analytics
- Application Infrastructure & Middleware - Data Exchange & Integration
- Functional Applications - Remote Monitoring & Control Systems
Applicable Industries
- Healthcare & Hospitals
- Life Sciences
Applicable Functions
- Product Research & Development
- Quality Assurance
Use Cases
- Predictive Quality Analytics
- Process Control & Optimization
- Remote Control
Services
- Software Design & Engineering Services
- System Integration
About The Customer
Oxford Biomedica is a world leader and pioneer in lentiviral vector research, manufacture, and clinical development. With over 20 years of experience in gene and cell therapy, they were the first organization to treat humans with in vivo lentiviral-based vectors. Their unique LentiVector® platform has led to a valuable portfolio of gene and cell therapy product candidates for oncological, ophthalmological, and CNS indications. In 2018, they started working with Antha to automate highly complex experiments, choosing Synthace and Antha for their rapid and flexible design and in silico simulation capabilities.
The Challenge
Oxford Biomedica faced the challenge of improving the efficiency and robustness of their in-house lentiviral vector production. The complexity of the biological system required a multifactorial experimental approach, which would have been very challenging to execute manually due to the number of experimental runs and the complexity of the design. Additionally, the high cost of therapy and the global demand for quality lentiviral vectors created a 'vector crunch' in the sector, necessitating the optimization of product quality, developability, and vector titres.
The Solution
Oxford Biomedica employed Antha in their R&D labs to optimize their transfection reagent mixes. Antha allowed them to save up to 40 hours on experimental planning and execution by automating the process through a liquid handling platform. The initial optimization identified transfection conditions that achieved a 3 to 10-fold increase in vector titre upon transduction, with an 81% reduction in pure error. Using Antha's DoE suite, they could rapidly define an automated liquid handling protocol, saving 95-98% of the time compared to manual methods. Antha's features also provided a 22% saving in liquid handling actions and a 32% saving in pipette tip usage.
Operational Impact
Quantitative Benefit
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